Evaluation of Blood, Buccal Swabs, And Hair Follicles for DNA Profiling Technique Using STR Markers
نویسندگان
چکیده
AIM To study the short tandem repeat (STR) pattern of DNA from the blood, buccal swabs, and hair follicles of the recipients of allogenic hematopoietic stem cell transplantation to examine whether these tissues contain donor derived cells. METHODS The study enrolled 25 patients who sustained engraftment. Peripheral blood, buccal swabs, and hair follicles were collected on days 21-30, 90, and 180 after transplantation and the chimeric status of the recipients was evaluated. RESULTS Donor derived cells existed in the blood and buccal swabs, but not in hair follicles, which can be used to obtain the pre-transplant sample of the recipient after transplant. CONCLUSION Peripheral blood and buccal swab do not serve as a reliable source of recipient's origin for DNA analysis of individuals who underwent allogenic hematopoietic stem cell transplantation at least within 6 months after transplant.
منابع مشابه
DNA profiling in blood, buccal swabs, and hair follicles of transplantation patients.
BACKGROUND/AIM Peripheral blood DNA profiles are often used in forensic identification and paternity investigations. Currently, because of the increasing number of patients who undergo transplantation, determining the history of transplantation has gained importance in forensic cases. The aim of this study was to investigate the potential of genetic chimerism analysis of blood, hair follicle, a...
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